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Tuesday, May 12, 2020 | History

2 edition of Purification and characterization Clostridium difficile toxin A found in the catalog.

Purification and characterization Clostridium difficile toxin A

Bruce E Urtz

Purification and characterization Clostridium difficile toxin A

by Bruce E Urtz

  • 267 Want to read
  • 3 Currently reading

Published .
Written in English

    Subjects:
  • Clostridium difficile,
  • Microbial toxins

  • Edition Notes

    Statementby Bruce E. Urtz
    The Physical Object
    Paginationiv, 35 leaves :
    Number of Pages35
    ID Numbers
    Open LibraryOL16266231M

    Toxin preparations were obtained by growing Clostridium difficile VPI strain in 2-liter brain heart infusion dialysis flasks at 37°C for 3 days. Theinitial step of the purification scheme involved ultrafiltration through an XM by: Toxin A (Clostridium difficile) Cytotoxic and enterotoxic: ALXC µg: Rho-glucosylating Clostridium difficile toxins A and B: new insights into structure and function: Purification and characterization of Clostridium difficile toxin: R. D. Rolfe & .

    The toxin of Clostridium difficile is considered responsible for pseudomembranous colitis which may follow antibiotic chemotherapy, This toxin can be produced in broth culture. After purification by gel filtration, ammonium sulfate precipitation, and ion-exchange chromatography, picograms protein was cytotoxic to Chinese hamster ovary by: 1.   Phelps CJ, Lyerly DL, Johnson JL, Wilkins TD. Construction and expression of the complete Clostridium difficile toxin A gene in Escherichia coli. Infect Immun. Jan; 59 (1)– [PMC free article] Popoff MR. Purification and characterization of Clostridium sordellii lethal toxin and cross-reactivity with Clostridium difficile by:

    Abstract. The ability of anaerobic bacteria to cause disease in humans and animals has been recognized for many years. The clostridia, for example, are well known for their ability to cause disease by producing a variety of toxins ranging from the potent neurotoxins of Clostridium tetani and Clostridium botulinum to the tissue-damaging toxins of the gas gangrene by: 1.   Purification and characterization of the lethal toxin (alpha-toxin) of Clostridium septicum. J Ballard, A Bryant, D Stevens, and R K Tweten Department of Microbiology and Immunology, University of Oklahoma Health Sciences Center, Oklahoma City


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Purification and characterization Clostridium difficile toxin A by Bruce E Urtz Download PDF EPUB FB2

Toxin preparations were obtained by growing Clostridium difficile VPI strain in 2-liter brain heart infusion dialysis flasks at 37 degrees C for 3 days. The initial step of the purification scheme involved ultrafiltration through an XM membrane filter.

Two toxic activities, designated toxins A and B Cited by: Purification and characterization of Clostridium difficile toxin. This article has been cited by other articles in PMC.

Abstract. Recent evidence indicates that toxigenic Clostridium difficile strains are a major cause of antimicrobial-associated ileocecitis in laboratory animals and pseudomembranous colitis in Cited by:   Recent evidence indicates that toxigenic Clostridium difficile strains are a major cause of antimicrobial-associated ileocecitis in laboratory animals and pseudomembranous colitis in humans.

difficile ATCC was cultivated in a synthetic medium to Cited by: toxin (ngens, C. septicum, C. histolyticum, C. oedematiens, andC.

sordellii) (kindly provided byE. Seligmann,FoodandDrugAdministration, Bureauof Biologics, Rockville, Md.). Toxin purification. Broth filtrate from a h culture of C. difficile ATCCgrown in BMP broth, wasprepared andsubjected to pressure ultra-filtration at 40C Cited by: C.

difficile ATCC was cultivated in a synthetic medium to which 3% ultrafiltrated proteose peptone was added. Purification of the toxin from broth filtrate was accomplished through ultrafiltration (, nominal-molecular-weight-limit membrane), precipitation with 75% (NH4)2SO4, and chromatographic separation using Bio-Gel A 5m followed Author: R D Rolfe and S M Finegold.

Hemorrhagic toxin (toxin HT) was purified from Clostridium sordellii culture filtrate. The purification steps included ultrafiltration through an XM membrane filter and immunoaffinity chromatography, using a monoclonal antibody to toxin A of Clostridium difficile as the ligand.

Purification and characterization of toxin B from strain of Clostridium difficile that does not produce toxin A Article (PDF Available) in Journal of Medical Microbiology 35(1) August Toxin preparations were obtained by growing Clostridium difficile VPI strain in 2-liter brain heart infusion dialysis flasks at 37 degrees C for 3 days.

The initial step of the purification scheme involved ultrafiltration through an XM membrane filter. Biochimica et Biophysica Acta, () Elsevier BBAPRO Production, purification and characterization of Clostridium d6fficile toxic proteins different from toxin A and from toxin B Javier F.

Torres 1,2 and Ivar LiSnnroth 1 t Department of Medz'cal Microbiology, Gothenburgh University, Giiteborg (Sweden) and 2 Unidad de lnvex'tigaci6n en Enfermedades Infecciosas y Cited by: Our study describes the production, purification, and properties of alpha-toxin from Clostridium novyi type A The bacterium produced maximal amounts of alpha-toxin when grown at 37 degrees C for 72 h in dialysis flask cultures containing brain heart infusion supplemented with % Tween 80 and 2% by:   Torres, J.

Purification and characterisation of toxin B from a strain of Clostridium difficile that does not produce toxin A. Med. Microbiol. 35, 40–44 () CAS ArticleCited by:   Purification and characterization of Clostridium sordellii lethal toxin and cross-reactivity with Clostridium difficile cytotoxin.

Popoff MR. Infect Immun, 55(1), 01 Jan Cited by: 76 articles | PMID: | PMCID: PMC Free to read. Purification and characterization of Clostridium sordellii hemorrhagic toxin and cross-reactivity with Clostridium difficile toxin A (enterotoxin).

Infect. Immun. –Cited by: Clostridium difficile toxin A is a toxin generated by Clostridium difficile. It is similar to Clostridium difficile Toxin B. The toxins are the main virulence factors produced by the gram positive, anaerobic, Clostridium difficile bacteria.

The toxins function by damaging the intestinal mucosa and cause the symptoms of C. difficile infection, including pseudomembranous colitis.

TcdA is one of the largest bacterial toxins Entrez: The toxins produced by Clostridium difficile share several functional properties with other bacterial toxins, like the heat-labile enterotoxin of Escherichia coli and cholera toxin. However, functional and structural differences also exist.

Like cholera toxin, their main target is the disruption of the microfilaments in the by: 9. Kamiya, P. Reed, and S. Borriello, Purification and characterisation of Clostridium difficile toxin A by bovine thyroglobulin affinity chromatography and dissociation in denaturing conditions with or without reduction, J.

Med. Microbiol. ().Cited by: 2. Production, purification and characterization of Clostridium difficile toxic proteins different from toxin A and from toxin B Article (PDF Available) in Biochimica et Biophysica Acta (2) Purification of toxin HT.

Toxin HT was specifically and quantitatively removedfromthe concentrated culture filtrate by the MAbto toxin Aof C. difficile coupled to Affi-Gel 10 (Fig. The toxin was eluted with 5 MMgCI2 6H20and showed a single immunoprecipitin arc by crossed immuno-electrophoresis against antiserum to C.

sordellii culture filtrate (Fig. lc).Cited by: The cytotoxin, also named toxin B, was isolated from a toxigenic strain of Clostridium difficile, purified to homogeneity and partially characterized.

The purification procedure included ultrafiltration followed by anion-exchange chromatography. We noticed that a non-specific nucleic material eluted with the protein during the by: A total of human clinical isolates of Clostridium difficile from all Australian states were screened for A−B+ strains by toxin gene PCR assays.

Nine ( %) strains were confirmed to be A−B+ by enzyme immunoassay for toxin production. Of these, six ( %) were binary toxin-positive by PCR. Using PCR ribotyping and toxinotyping, the A−B+ strains could be grouped into seven ribotypes Cited by:.

Purification and characterization of toxin B from Clostridium difficile Article (PDF Available) in Infection and Immunity 56(7) August with 11 Reads How we measure 'reads'.Toxin B from Clostridium difficile was purified to homogeneity and characterized.

Purification of toxin B was achieved by gel filtration, chromatography on two consecutive anion-exchange columns, and chromatography on a high-resolution anion-exchange column in the presence of 50 mM by: This organism is not normally present in the colonic flora of adults, and the source of C.

difficile in PMC is unknown. What is known is that exposure to certain antimicrobials appears to allow this organism to flourish, produce toxin and cause colitis. Most pathogenic clostridia produce more than one toxin, Cited by: